SERtool User Guide

This tool analyzes enriched regions in biological sequences, supporting multiple input formats and analysis modes.

Quick Run: Analyze in 2 Steps

To preview the output format, click the blue Example Result button. The example page shows downloadable files and a link redirecting to STRING for functional enrichment analysis of detected regions.

Get started instantly — no need to adjust advanced settings.

  1. Input Pattern: Input the amino acid sequence to search.
  2. Click "Analyze Sequence": The tool will automatically use optimized default settings (Human, Fixed Mode with point 1(18,20), point 2 (30,50)) to scan and detect enriched regions.

The algorithm's principles and example statistics on the number of detected regions are shown below.

Algorithm workflow and result statistics example

Analysis Mode Setting

Fixed mode is faster than formula mode in some conditions because it only calculates one or two fixed points. (X = not required).

Condition Example Start End Step Mode Point(hit,window) Note
Single Class Amino Acid Enrichment D-E 20 100 1 Formula (18,20)(30,50) DDDDEEEEEE
Fixed Sequence Enrichment DE 20 100 1 Formula (9,20)(15,50) DEDEDEDEDE
Wildcard Containing Enrichment H.K 20 100 1 Formula (4,20)(8,50) HTKHSK
Continuous Sequence Enrichment R X X 1 Fix (9,9) RRRRRRRRR
Motif Sequence KDEL X X 1 Fix (1,20) KDEL

Score Mode

Set a score threshold; the system reports all regions with scores ≥ threshold.

Suitable for cases where functional region scoring criteria are known.

Proportion Mode

Set a rate threshold; the system reports all regions with proportion ≥ threshold.

Suitable for cases where functional region scoring criteria are known.

📄 Citing SERtool

Please cite the following publication when using this tool or dataset:

Ren Meng, .etc. (2025). SERtool: A Sequence Analysis Platform Augmented with Mutation-Induced Protein Sequences.[Journal Name], [Volume](Issue), pp. [Page Range]. https://doi.org/[DOI]